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The optical signal is strongly present in the course of the first 5 hours submit injection (panel 2–5), decreases over time (panel 6–8), but continues to be present after 21 days . In panel 10 is shown the bioluminescence acquire vary. RT-PCR demonstrates the presence of the luciferase gene in 5 injected kidneys, in contrast with the cells before injection and in non-injected kidney . Immuno-fluorescence staining of injected kidney with hAFSC after 3 weeks. The purple fluorescence confirms the presence of hAFSC expressing luciferase.
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In Figure 3B is shown an in vitro experiment to find out the minimal number (1×105) of cells that exhibited a readily detectable optical sign. In Figure 3C is proven stay imaging of a proper kidney injected with 1,2×106 hAFSC after damage induction. The animal injected with luciferin only did not show any signal , whereas the lucierase fluorescent signal is clearly evident, and even spreads into a number of zones of the physique, such because the lung over the first few hours (panels 2–3).
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